Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus S19 and RB51 and Brucella melitensis Rev1
نویسندگان
چکیده
منابع مشابه
Detection of Brucella melitensis and Brucella abortus strains using a single-stage PCR method
Brucella melitensis and Brucella abortus are of the most important causes of brucellosis, an infectious disease which is transmitted either directly or indirectly including consuming unpasteurized dairy products. Both strains are considered endemic in Iran. Common diagnostic methods such as bacteriologic cultures are difficult and time consuming regarding the bacteria. The aim of this study w...
متن کاملDevelopment of a multiplex polymerase chain reaction assay for differentiation of field strain isolates and vaccine strains S19 and RB51 of Brucella in Iran
Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. Since brucellosis eradication programme in Iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. We developed and evaluated a multiplex polyme...
متن کاملDifferentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR.
Several PCR assays which identify the genus Brucella but do not discriminate among species have been reported. We describe a PCR assay that comprises five oligonucleotide primers which can identify selected biovars of four species of Brucella. Individual biovars within a species are not differentiated. The assay can identify three biovars (1, 2, and 4) of B. abortus, all three biovars of B. mel...
متن کاملDevelopment of a Differential PCR Assay for Detection of Brucella abortus and Brucella melitensis: an Analytical Approach for Monitoring of Brucella spp. in Foods of Animal Origin
Background: Classical bacteriological detection of Brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. However, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. Methods: Panel of some reference strains belonging to ...
متن کاملA Novel PCR Assay for Detecting Brucella abortus and Brucella melitensis
OBJECTIVES Brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. The classical bacteriological detection process used to identify Brucella spp. is difficult and time-consuming. This study aimed to develop a novel molecular assay for detecting brucellosis. METHODS All complete sequences of chromosome 1 with 2.1-Mbp lengths were compared among all avai...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2006
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2005.062596